ce design vazyme

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     · 15,Vazyme,PCR。DH5α(Vazyme #C502),XL10(Vazyme #C503),Fast-T1(Vazyme #C505),。

  • CE Design V1.03(

     · CE Design V1.03(ClonExpress Mut Express) CE Design V1.03 Windows XP VB 6.0 ,

  • How to Design a PrimerFujian Agriculture and Forestry

     · Primer design for PCR- recombination enzyme system For recombination enzyme system primer design, in our example, we will use C112 (Vazyme) kit as a model. The basic PCR primers for recombination cloning consist of Recombination Sequence 15bp homologous sequence of the backbone at the upstream and downstream of the cloning site.

  • Q113 AceQ Probe U

     · Catalog#Q113 Vazymebiotechco.,ltd. Introduction AceQU ProbeMasterMix The AceQ U Probe Master Mix is designed for probe-based qPCR. The hot-start AceTaq DNA Polymerase (Vazyme, #P401) and an optimized buffer significantly improve its sensitivity and specificity for qPCR.

  • A multi‐functional genetic manipulation system and its use

     · All of the primers used in recombinant plasmid construction were automatically designed by CE Design Version 1.04 software (Vazyme). The counter-selection system AACC was constructed by replacing the AOX1 expression cassette in pPICZαA plasmid with an URA3 expression cassette, which was amplified form the genome of P. pastoris.

  • AceQSYBR qPCRMasterMix

     · Vazyme biotech co., ltd. Catalog#Q111 Introduction AceQSYBR ®qPCRMasterMix The Vazyme AceQ SYBR ® qPCR Master Mix utilizes a special performance-enhanced AceTaq DNA Polymerase (Vazyme, #P401) protected via a chemically- modified hot-start activation technique to perform SYBR Green I based quantitative PCR (qPCR).

  • STAU1 selectively regulates the expression of inflammatory

     · CE Design 1.04 software (Vazyme Biotech Co., Ltd.). was employed to design primers for Hot Fusion . There was a gene-specific sequence and a pIRES-hrGFP-1a vector (part no. 240031 Agilent Technologies, Inc.) sequence (17–30 bp) in each primer.

  • ClonExpressIIOneStepCloningkitCellagen Tech

     · VazymeBiotechCo.,Ltd Order [email protected] Support [email protected] vazyme Forresearchuseonly,notforuseindiagnosticprocedures. M DL5,000Marker.

  • ClonExpress®Ultra One Step Cloning Kit(

     ·  CE Design 1. PCR,5’3’(1520 bp)。2×ClonExpress ® Mix,50℃5-15 min,。

  • RBM4 modulates the proliferation and expression of

     · Primer pairs used for hot fusion were designed with CE Design V1.04 (Vazyme). Each of the primers comprises a fragment of RBM4-specific sequences and a 17–30 bp sequence of the pIRES-hrGFP-1a vector (Stratagene). The pIRES-hrGFP-1a vector

  • CE Design V1.03.rar__

     ·  Vazyme ¥ / CE Design V1.03.rar 291 10 29 CE Design V1.03.rar

  • PbCSE1 promotes lignification during stone cell

     · CE Design V1.03 software was used to design the primer pairs containing Xba I and BamH I restriction sites among the pCAMBIA1300 vector. (VAZYME, Nanjing, China) to clone the target gene

  • Tm Calculatorcrm.vazyme

     · Tm Calculator. * Please enter the primer sequence (direction is from 5' to 3', non-ATCG characters will be automatically filtered) 0.

  • ClonExpress II One Step Cloning Kit (

     · ClonExpress II One Step Cloning Kit ()(C112)ClonExpress® II One Step Cloning Kit、、,

  • Mut Express II Fast Mutagenesis Kit V2_

    Mut Express ® II Fast Mutagenesis Kit V2 ClonExpress ® 。 ,DpnI 、ClonExpress ® 。 Phanta ® Max Super-Fidelity DNA Polymerase ClonExpress ® 。

  • Vazyme Biotech Co.,Ltd

    Vazyme Biotech Co.,Ltd-Vazyme Biotech is devoted to the development and production of enzymes and antibodies. Our products cover clinical diagnosis, molecular diagnostics, high-throughput sequencing and life science research and other related fields.

  • ClonExpress II One Step Cloning Kit

     · ,, >95%. CE Design. ClonExpress®、 DNA ,。. ClonExpress®II , Exnase®II。.

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • The synergistic effect of NELL1 and adipose-derived stem

     · The primers were designed by CE Design (Vazyme, China). Then the cloned Nell1 fragment was inserted into the linearized vector pCDH-CMV-MCS-EF1-copGFP by ClonExpress II One Step Cloning Kit (Vazyme, China) to construct pCDH-Nell1-copGFP (lenti-Nell1) vector.

  • miRNA1stStrandcDN A SynthesisKit(bystem-loop)

     · sequence based on the miRNA sequence.Reverse transcription and qPCR primer design can also be performed using the primer design softwaremiRNADesign(freedownload vazyme),whichcan beusedtoobtainprimersequences bysimplyinputtingthe

  • Single-Fragment Cloning (ClonExpress II)crm.vazyme

     · Primer Design for ClonExpress Entry. Single-Fragment Cloning. Single-Fragment Cloning (ClonExpress II) Multi-Fragment Assembly. Generate CE II Amplification Primers Empty . 5'--3' 5'--3' * Select the restriction enzyme cleavage site that borders the 3' end of the insert

  • Multi-Fragment Assembly (ClonExpress MultiS)crm.vazyme

     · Multi-Fragment Assembly (ClonExpress MultiS) * Please enter the complete sequence of the cloning vector or the sequence near the cloning site (5' to 3', and the upstream and downstream sequences of the cloning site are at least 20 bp each) 0. * Select the

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • An Efficient Virus-Induced Gene Silencing System for

     · The fragments were amplified with primers designed by the software of CE Design (Vazyme, Nanjing, China Supplementary Table S2). 4 The pTRV2 vector was then digested with restriction endonuclease BamHI and XhoI (TAKARA, Japan). 5 A Gel Extraction Kit D2500 (Omega Biotek) 5 was used to purify the PCR product and the digested pTRV2 vector.

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • Sales Proposal of Vazyme’s Solutions for Coronavirus

     · 1. About Vazyme 2. Vazyme Solution for Coronavirus Detection 3. Summary (Price / Lead Time / Production Capacity / Contact) 4. 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold-Based) a. Product Information / Features / Pictures / Package b. Brochure c. Instruction for Use d. Certificates (FDA, CEIVD, ISO 13485, CFDA) e. Clinical Test Report

  • ClonExpress II One Step Cloning Kit_——

    ClonExpress II One Step Cloning Kit-——-ClonExpress®IIOneStepCloningKit 、、,50bp-10kbDNA