exnase vazyme

  • Rapid Cloning _Product_Vazyme Biotech Co.,Ltd

    Rapid Cloning -Vazyme Biotech Co.,Ltd. ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, which can quickly and efficiently clone

  • Role refinement of melanin synthesis genes by gene

     · The knockout cassettes were constructed using recombinase Exnase (Vazyme Biotech, China) to insert one 1.0–1.2 kb upstream fragment and one 1.0–1.2 kb downstream fragment of targeted genes and a sulfonylurea resistance gene (SUR) into a SalI and HindIII-linearized vector, pKO3B, which contains a suicide gene, HSVtk (Yan et al., 2019).

  • ClonExpress II One Step Cloning Kit_Vazyme Biotech Co.,Ltd

    The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps.

  • ClonExpress II One Step Cloning Kit_——

    ClonExpress®、 DNA ,。. ClonExpress®II , Exnase®II。. ; / PCR 5’ , PCR 5’ 3’ (15 bp-20 bp)。.

  • ClonExpress MultiS

     · ClonExpress MultiS.. Vazyme biotech co., ltd. vazyme /Web vazyme /Tel /Sales [email protected] /Support [email protected] /Service [email protected] Vazyme Biotech Co., Ltd

  • TcpA, a novel Yersinia ruckeri TIR-containing virulent

     · The tcpA constructs were obtained from SC09 genomic DNA by recombinase Exnase II (ClonExpress II, Vazyme) and then cloned in pCMV-Tag 2B. 2.9. Co-immunoprecipitations (CO-IP) Rainbow trout head kidney macrophages were infected with the wild-type SC09 for 12 h. Cells were washed twice in ice-cold PBS, harvested and cell lysis buffer was added.

  • Identification of a unique 1,4-β- d -glucan glucohydrolase

     · Abstract Cytophaga hutchinsonii is an aerobic cellulolytic soil bacterium that rapidly digests crystalline cellulose. The predicted mechanism by which C. hutchinsonii digests cellulose differs from that of other known cellulolytic bacteria and fungi. The genome of C. hutchinsonii contains 22 glycoside hydrolase (GH) genes, which may be involved in cellulose degradation. One predicted GH with

  • ClonExpressIIOneStepCloningkitCellagen Tech

     · VazymeBiotechCo.,Ltd Order [email protected] Support [email protected] vazyme Forresearchuseonly,notforuseindiagnosticprocedures. M DL5,000Marker.

  • Team PYMS GZ China/Lab notebook2020.igem

     · So we need to use Exnase II (C214, Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul 37℃ 30min

  • ClonExpress II One Step Cloning Kit

     · ClonExpress®II , Exnase®II。 ; / PCR 5’ , PCR 5’ 3’ (15 bp-20 bp)。

  • C112 ClonExpress II One Step Cloning kit

     · VazymeBiotechCo.,Ltd Order [email protected] m Support [email protected] m ww w.vazyme m Forresearchuseon ly,notforuseindiagnosticprocedures. M DL5,000Marker.

  • ClonExpress II One Step Cloning Kit (

     · ClonExpress II One Step Cloning Kit ()(C112)ClonExpress® II One Step Cloning Kit、、,

  • ClonExpress II One Step Cloning Kit #C112

    ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is

  • Team SJTU-BioX-Shanghai/Experiments2016.igem

     · As for the ligation process, we use the T4 ligase from Thermo Scientific (#EL0011) or Exnase from Vazyme (C112-01/02). Ligation using T4 ligase. All of the ligation process we did using T4 ligase were between sticky ends, the reaction mixture was as following

  • ClonExpress MultiS One Step Cloning Kit _

    ClonExpress® MultiS,bufferExnase® MultiS。. ,PCR5'-;PCR,Exnase®,30 min,,95%。. ,

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    C115-01/02. ClonExpress®UltraOneStepCloningKit 5-15min; Mix,; 50bp~10kb,>95%; 、、; 、/; PCR,5’3’. 800.00.

  • Molecular Cloning/Mutagenesis_Product_Vazyme Biotech

    Molecular Cloning/Mutagenesis-Vazyme Biotech Co.,Ltd. ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector.

  • VazymeStratech

    Vazyme Biotech is devoted to the development and production of enzymes and antibodies. Their products cover clinical diagnosis, molecular diagnostics, high-throughput sequencing and life science research and other related fields. With advanced capability of R&D and cutting-edge technology, Vazyme Biotech will create a new pattern of National

  • ClonExpress

     · ClonExpress. Vazyme biotech co., ltd. vazyme /Web vazyme /Tel /Sales [email protected] /Support [email protected] /Service [email protected] Vazyme Biotech Co., Ltd Version 3.2 ClonExpress TM II

  • ftp.polgen.pl

     · Compared to the previous version, Exnase® II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even using low efficient competent cells (107 cfu/μg). Additionally, Exnase® II is also compatible with the endonuclease digesting reaction and PCR reaction assay.

  • SARS-CoV-2 nucleocapsid protein phase separates with

     · DNA fragments encoding human ubiquitin-specific peptidase 10 (USP10), double-stranded RNA-dependent protein kinase (PKR), and G3BP2 were PCR-amplified from cDNA that was reverse transcribed from HEK293T total RNA with reverse transcriptase (Vazyme, Nanjing, China) according to the manufacturer’s instructions.

  • C113 ClonExpress MultiS One Step Cloning Kit

     · VazymeBiotechCo.,Ltd. Order [email protected] Support [email protected] vazyme Forresearchuseonly,notforuseindiagnosticprocedures. Inserts can be amplifiedby any polymerase (i.e. Taq DNA polymerase or high-fidelitypolymerase). It will not interfere with the recombina-

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • Mut Express MultiS Fast Mutagenesis Kit V2_Vazyme Biotech

    Mut Express MultiS Fast Mutagenesis Kit V2-Vazyme Biotech Co.,Ltd- Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the

  • Enzyme and Microbial Technologyjiangnan.edu.cn

     · resultant PCR products were ligated by Exnase II (Vazyme Co. Ltd) into pET28a double-digested with BamH I and Xho I, and transformed into E. coli BL21(DE3). Positive transformants, evaluated by colony PCR and Fig. 1. Screening results of CHCM hydrolase-producing strains.

  • Homozygous mutations in C14orf39/SIX6OS1 cause non

     · The construction of the plasmids was achieved by homologous recombination with the Exnase enzyme (Vazyme, C113). Yeast transformation was conducted according to the manual (PT1172-1) from Clontech Laboratories,

  • Team SJTU-BioX-Shanghai/Experiments2016.igem

     · As for the ligation process, we use the T4 ligase from Thermo Scientific (#EL0011) or Exnase from Vazyme (C112-01/02). Ligation using T4 ligase. All of the ligation process we did using T4 ligase were between sticky ends, the reaction mixture was as following

  • C114Vazyme Biotech Co.,Ltd

    MedicalExpo()。 C114, Vazyme Biotech Co.,Ltd。,。,MedicalExpo。

  • ClonExpress MultiS One Step Cloning Kit C113

    Product Description. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit, up to 5 fragments can be orderly assembled in a single

  • ClonExpress MultiS One Step Cloning Kit (

     · ClonExpress MultiS One Step Cloning Kit ()(C113)ClonExpress® MultiS One Step Cloning Kit、、,

  • ClonExpress

     · ClonExpress.pdf,ClonExpressTM II One Step Cloning Kit Vazyme Biotech Co., Ltd w w /Web w . v /Tel a Vazyme biotech co., ltd. z y /Sales [email protected] m e . c